Coding

Part:BBa_K3332025:Design

Designed by: Zhichun Yang   Group: iGEM20_XMU-China   (2020-10-17)


phnJ_mut21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 549
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 597
    Illegal AgeI site found at 268
    Illegal AgeI site found at 775
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Put the one with best activity in the final circuit with phn series, RNAi circuits with best activity and kill switch.


Source

Point mutation

References